ARHGEF1

ARHGEF1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1IAP, 1SHZ, 3AB3, 3ODO, 3ODW, 3ODX, 3P6A
Identifiers
Aliases	ARHGEF1, GEF1, LBCL2, LSC, P115-RHOGEF, SUB1.5, Rho guanine nucleotide exchange factor 1
External IDs	MGI: 1353510 HomoloGene: 3454 GeneCards: ARHGEF1
Gene ontology Molecular function • Rho guanyl-nucleotide exchange factor activity • poly(A) RNA binding • protein binding • GTPase activator activity • guanyl-nucleotide exchange factor activity Cellular component • cytoplasm • cytosol • plasma membrane • membrane Biological process • negative regulation of axonogenesis • cell proliferation • regulation of Rho protein signal transduction • Rho protein signal transduction • positive regulation of GTPase activity Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9138	16801
Ensembl	ENSG00000076928	ENSMUSG00000040940
UniProt	Q92888	Q61210
RefSeq (mRNA)	NM_004706 NM_198977 NM_199002	NM_001130150 NM_001130151 NM_001130152 NM_001130153 NM_008488
RefSeq (protein)	NP_004697.2 NP_945328.1 NP_945353.1	NP_001123622.1 NP_001123623.1 NP_001123624.1 NP_001123625.1 NP_032514.1
Location (UCSC)	Chr 19: 41.88 – 41.93 Mb	Chr 7: 24.9 – 24.93 Mb
PubMed search	[1]	[2]
Wikidata

View/Edit Human

View/Edit Mouse

Rho guanine nucleotide exchange factor 1 is a protein that in humans is encoded by the ARHGEF1 gene.^[3][4][5]

Function

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.^[5]

Interactions

ARHGEF1 has been shown to interact with:

• CD44,^[6]
• GNA12,^[6]
• GNA13,^[6][7][8] and
• P110α^[9]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Hart MJ, Sharma S, elMasry N, Qiu RG, McCabe P, Polakis P, Bollag G (Nov 1996). "Identification of a novel guanine nucleotide exchange factor for the Rho GTPase". J Biol Chem. 271 (41): 25452–8. doi:10.1074/jbc.271.41.25452. PMID 8810315.
4. Aasheim HC, Pedeutour F, Smeland EB (May 1997). "Characterization, expression and chromosomal localization of a human gene homologous to the mouse Lsc oncogene, with strongest expression in hematopoetic tissues". Oncogene. 14 (14): 1747–52. doi:10.1038/sj.onc.1200994. PMID 9135076.
5. "Entrez Gene: ARHGEF1 Rho guanine nucleotide exchange factor (GEF) 1".
6. Johnson EN, Seasholtz TM, Waheed AA, Kreutz B, Suzuki N, Kozasa T, Jones TL, Brown JH, Druey KM. "RGS16 inhibits signalling through the G alpha 13-Rho axis". Nat. Cell Biol. 5 (12): 1095–103. doi:10.1038/ncb1065. PMID 14634662.
7. Bhattacharyya R, Wedegaertner PB (Apr 2003). "Mutation of an N-terminal acidic-rich region of p115-RhoGEF dissociates alpha13 binding and alpha13-promoted plasma membrane recruitment". FEBS Lett. 540 (1-3): 211–6. doi:10.1016/s0014-5793(03)00267-9. PMID 12681510.
8. Hart MJ, Jiang X, Kozasa T, Roscoe W, Singer WD, Gilman AG, Sternweis PC, Bollag G (Jun 1998). "Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13". Science. 280 (5372): 2112–4. doi:10.1126/science.280.5372.2112. PMID 9641916.
9. Holinstat M, Mehta D, Kozasa T, Minshall RD, Malik AB (Aug 2003). "Protein kinase Calpha-induced p115RhoGEF phosphorylation signals endothelial cytoskeletal rearrangement". J. Biol. Chem. 278 (31): 28793–8. doi:10.1074/jbc.M303900200. PMID 12754211.

Further reading

• Fukuhara S, Chikumi H, Gutkind JS (2001). "RGS-containing RhoGEFs: the missing link between transforming G proteins and Rho?". Oncogene. 20 (13): 1661–8. doi:10.1038/sj.onc.1204182. PMID 11313914.
• Kozasa T, Jiang X, Hart MJ, Sternweis PM, Singer WD, Gilman AG, Bollag G, Sternweis PC (1998). "p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13". Science. 280 (5372): 2109–11. doi:10.1126/science.280.5372.2109. PMID 9641915.
• Hart MJ, Jiang X, Kozasa T, Roscoe W, Singer WD, Gilman AG, Sternweis PC, Bollag G (1998). "Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13". Science. 280 (5372): 2112–4. doi:10.1126/science.280.5372.2112. PMID 9641916.
• Zhang H, Wang L, Kao S, Whitehead IP, Hart MJ, Liu B, Duus K, Burridge K, Der CJ, Su L (1999). "Functional interaction between the cytoplasmic leucine-zipper domain of HIV-1 gp41 and p115-RhoGEF". Curr. Biol. 9 (21): 1271–4. doi:10.1016/S0960-9822(99)80511-9. PMID 10556093.
• Kim ST, Lim DS, Canman CE, Kastan MB (2000). "Substrate specificities and identification of putative substrates of ATM kinase family members". J. Biol. Chem. 274 (53): 37538–43. doi:10.1074/jbc.274.53.37538. PMID 10608806.
• Bhattacharyya R, Wedegaertner PB (2000). "Galpha 13 requires palmitoylation for plasma membrane localization, Rho-dependent signaling, and promotion of p115-RhoGEF membrane binding". J. Biol. Chem. 275 (20): 14992–9. doi:10.1074/jbc.M000415200. PMID 10747909.
• Kozasa T (2001). "Regulation of G protein-mediated signal transduction by RGS proteins". Life Sci. 68 (19–20): 2309–17. doi:10.1016/S0024-3205(01)01020-7. PMID 11358341.
• Wells CD, Gutowski S, Bollag G, Sternweis PC (2001). "Identification of potential mechanisms for regulation of p115 RhoGEF through analysis of endogenous and mutant forms of the exchange factor". J. Biol. Chem. 276 (31): 28897–905. doi:10.1074/jbc.M102913200. PMID 11384980.
• Chen Z, Wells CD, Sternweis PC, Sprang SR (2001). "Structure of the rgRGS domain of p115RhoGEF". Nat. Struct. Biol. 8 (9): 805–9. doi:10.1038/nsb0901-805. PMID 11524686.
• Park B, Nguyen NT, Dutt P, Merdek KD, Bashar M, Sterpetti P, Tosolini A, Testa JR, Toksoz D (2003). "Association of Lbc Rho guanine nucleotide exchange factor with alpha-catenin-related protein, alpha-catulin/CTNNAL1, supports serum response factor activation". J. Biol. Chem. 277 (47): 45361–70. doi:10.1074/jbc.M202447200. PMID 12270917.
• Driessens MH, Olivo C, Nagata K, Inagaki M, Collard JG (2002). "B plexins activate Rho through PDZ-RhoGEF". FEBS Lett. 529 (2–3): 168–72. doi:10.1016/S0014-5793(02)03323-9. PMID 12372594.
• Chen Z, Singer WD, Wells CD, Sprang SR, Sternweis PC (2003). "Mapping the Galpha13 binding interface of the rgRGS domain of p115RhoGEF". J. Biol. Chem. 278 (11): 9912–9. doi:10.1074/jbc.M212695200. PMID 12525488.
• Bhattacharyya R, Wedegaertner PB (2003). "Characterization of G alpha 13-dependent plasma membrane recruitment of p115RhoGEF". Biochem. J. 371 (Pt 3): 709–20. doi:10.1042/BJ20021897. PMC 1223324. PMID 12534370.
• Bhattacharyya R, Wedegaertner PB (2003). "Mutation of an N-terminal acidic-rich region of p115-RhoGEF dissociates alpha13 binding and alpha13-promoted plasma membrane recruitment". FEBS Lett. 540 (1–3): 211–6. doi:10.1016/S0014-5793(03)00267-9. PMID 12681510.
• Bourguignon LY, Singleton PA, Zhu H, Diedrich F (2003). "Hyaluronan-mediated CD44 interaction with RhoGEF and Rho kinase promotes Grb2-associated binder-1 phosphorylation and phosphatidylinositol 3-kinase signaling leading to cytokine (macrophage-colony stimulating factor) production and breast tumor progression". J. Biol. Chem. 278 (32): 29420–34. doi:10.1074/jbc.M301885200. PMID 12748184.
• Holinstat M, Mehta D, Kozasa T, Minshall RD, Malik AB (2003). "Protein kinase Calpha-induced p115RhoGEF phosphorylation signals endothelial cytoskeletal rearrangement". J. Biol. Chem. 278 (31): 28793–8. doi:10.1074/jbc.M303900200. PMID 12754211.