ARHGEF7

ARHGEF7
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1BY1, 1ZSG, 2L3G
Identifiers
Aliases	ARHGEF7, BETA-PIX, COOL-1, COOL1, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB, Rho guanine nucleotide exchange factor 7
External IDs	MGI: 1860493 HomoloGene: 2895 GeneCards: ARHGEF7
Gene ontology Molecular function • protein binding • protein kinase binding • guanyl-nucleotide exchange factor activity • Rho guanyl-nucleotide exchange factor activity Cellular component • cytoplasm • cytosol • cell projection • focal adhesion • ruffle • plasma membrane • protein complex • cell junction • neuronal cell body • cell cortex • neuron projection • storage vacuole • lamellipodium • intracellular Biological process • regulation of GTP binding • intracellular signal transduction • negative regulation of epidermal growth factor receptor signaling pathway • positive regulation of lamellipodium morphogenesis • Golgi organization • ephrin receptor signaling pathway • nervous system development • focal adhesion assembly • positive regulation of GTPase activity • positive regulation of substrate adhesion-dependent cell spreading • positive regulation of growth hormone secretion • hematopoietic progenitor cell differentiation • positive regulation of apoptotic process • positive regulation of protein binding • regulation of Rho protein signal transduction • regulation of small GTPase mediated signal transduction • positive regulation of fibroblast migration • signal transduction • lamellipodium assembly Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	8874	54126
Ensembl	ENSG00000102606	ENSMUSG00000031511
UniProt	Q14155	Q9ES28
RefSeq (mRNA)	NM_001113511 NM_001113512 NM_001113513 NM_003899 NM_145735 NM_001320851 NM_001320852 NM_001320853 NM_001320854	NM_001113517 NM_001113518 NM_017402
RefSeq (protein)	NP_001106983.1 NP_001106984.1 NP_001106985.1 NP_003890.1 NP_663788.1 NP_001307780.1 NP_001307782.1 NP_001307783.1 NP_001106985.1 NP_003890.1 NP_001307780.1	NP_001106989.1 NP_001106990.1 NP_059098.2
Location (UCSC)	Chr 13: 111.11 – 111.31 Mb	Chr 8: 11.73 – 11.84 Mb
PubMed search	[1]	[2]
Wikidata

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Rho guanine nucleotide exchange factor 7 is a protein that in humans is encoded by the ARHGEF7 gene.^[3][4][5]

Function

Rho GTPase play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein-coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein can induce membrane ruffling. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene, but some of their full-length sequences have not been determined.^[5]

Interactions

ARHGEF7 has been shown to interact with:

• Cbl gene,^[6]
• GIT1,^[7][8]
• SHANK1^[9]
• SHANK2,^[9] and
• SHANK3.^[9]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Oh WK, Yoo JC, Jo D, Song YH, Kim MG, Park D (Jul 1997). "Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion". Biochem Biophys Res Commun. 235 (3): 794–8. doi:10.1006/bbrc.1997.6875. PMID 9207241.
4. Bagrodia S, Taylor SJ, Jordon KA, Van Aelst L, Cerione RA (Oct 1998). "A novel regulator of p21-activated kinases". J Biol Chem. 273 (37): 23633–6. doi:10.1074/jbc.273.37.23633. PMID 9726964.
5. "Entrez Gene: ARHGEF7 Rho guanine nucleotide exchange factor (GEF) 7".
6. Flanders JA, Feng Q, Bagrodia S, Laux MT, Singavarapu A, Cerione RA (Aug 2003). "The Cbl proteins are binding partners for the Cool/Pix family of p21-activated kinase-binding proteins". FEBS Lett. 550 (1-3): 119–23. doi:10.1016/s0014-5793(03)00853-6. PMID 12935897.
7. Kim S, Ko J, Shin H, Lee JR, Lim C, Han JH, Altrock WD, Garner CC, Gundelfinger ED, Premont RT, Kaang BK, Kim E (Feb 2003). "The GIT family of proteins forms multimers and associates with the presynaptic cytomatrix protein Piccolo". J. Biol. Chem. 278 (8): 6291–300. doi:10.1074/jbc.M212287200. PMID 12473661.
8. Bagrodia S, Bailey D, Lenard Z, Hart M, Guan JL, Premont RT, Taylor SJ, Cerione RA (Aug 1999). "A tyrosine-phosphorylated protein that binds to an important regulatory region on the cool family of p21-activated kinase-binding proteins". J. Biol. Chem. 274 (32): 22393–400. doi:10.1074/jbc.274.32.22393. PMID 10428811.
9. Park E, Na M, Choi J, Kim S, Lee JR, Yoon J, Park D, Sheng M, Kim E (May 2003). "The Shank family of postsynaptic density proteins interacts with and promotes synaptic accumulation of the beta PIX guanine nucleotide exchange factor for Rac1 and Cdc42". J. Biol. Chem. 278 (21): 19220–9. doi:10.1074/jbc.M301052200. PMID 12626503.

Further reading

• Matoba R, Okubo K, Hori N, Fukushima A, Matsubara K (1994). "The addition of 5'-coding information to a 3'-directed cDNA library improves analysis of gene expression". Gene. 146 (2): 199–207. doi:10.1016/0378-1119(94)90293-3. PMID 8076819.
• Nagase T, Seki N, Tanaka A, Ishikawa K, Nomura N (1996). "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (4): 167–74, 199–210. doi:10.1093/dnares/2.4.167. PMID 8590280.
• Manser E, Loo TH, Koh CG, Zhao ZS, Chen XQ, Tan L, Tan I, Leung T, Lim L (1998). "PAK kinases are directly coupled to the PIX family of nucleotide exchange factors". Mol. Cell. 1 (2): 183–92. doi:10.1016/S1097-2765(00)80019-2. PMID 9659915.
• Turner CE, Brown MC, Perrotta JA, Riedy MC, Nikolopoulos SN, McDonald AR, Bagrodia S, Thomas S, Leventhal PS (1999). "Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling". J. Cell Biol. 145 (4): 851–63. doi:10.1083/jcb.145.4.851. PMC 2133183. PMID 10330411.
• Bagrodia S, Bailey D, Lenard Z, Hart M, Guan JL, Premont RT, Taylor SJ, Cerione RA (1999). "A tyrosine-phosphorylated protein that binds to an important regulatory region on the cool family of p21-activated kinase-binding proteins". J. Biol. Chem. 274 (32): 22393–400. doi:10.1074/jbc.274.32.22393. PMID 10428811.
• Premont RT, Claing A, Vitale N, Perry SJ, Lefkowitz RJ (2000). "The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing". J. Biol. Chem. 275 (29): 22373–80. doi:10.1074/jbc.275.29.22373. PMID 10896954.
• Ku GM, Yablonski D, Manser E, Lim L, Weiss A (2001). "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT". EMBO J. 20 (3): 457–65. doi:10.1093/emboj/20.3.457. PMC 133476. PMID 11157752.
• Koh CG, Tan EJ, Manser E, Lim L (2002). "The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family". Curr. Biol. 12 (4): 317–21. doi:10.1016/S0960-9822(02)00652-8. PMID 11864573.
• Brown MC, West KA, Turner CE (2002). "Paxillin-dependent paxillin kinase linker and p21-activated kinase localization to focal adhesions involves a multistep activation pathway". Mol. Biol. Cell. 13 (5): 1550–65. doi:10.1091/mbc.02-02-0015. PMC 111126. PMID 12006652.
• Shin EY, Shin KS, Lee CS, Woo KN, Quan SH, Soung NK, Kim YG, Cha CI, Kim SR, Park D, Bokoch GM, Kim EG (2003). "Phosphorylation of p85 beta PIX, a Rac/Cdc42-specific guanine nucleotide exchange factor, via the Ras/ERK/PAK2 pathway is required for basic fibroblast growth factor-induced neurite outgrowth". J. Biol. Chem. 277 (46): 44417–30. doi:10.1074/jbc.M203754200. PMID 12226077.
• Rosenberger G, Jantke I, Gal A, Kutsche K (2003). "Interaction of alphaPIX (ARHGEF6) with beta-parvin (PARVB) suggests an involvement of alphaPIX in integrin-mediated signaling". Hum. Mol. Genet. 12 (2): 155–67. doi:10.1093/hmg/ddg019. PMID 12499396.
• Park E, Na M, Choi J, Kim S, Lee JR, Yoon J, Park D, Sheng M, Kim E (2003). "The Shank family of postsynaptic density proteins interacts with and promotes synaptic accumulation of the beta PIX guanine nucleotide exchange factor for Rac1 and Cdc42". J. Biol. Chem. 278 (21): 19220–9. doi:10.1074/jbc.M301052200. PMID 12626503.
• Mignone F, Grillo G, Liuni S, Pesole G (2003). "Computational identification of protein coding potential of conserved sequence tags through cross-species evolutionary analysis". Nucleic Acids Res. 31 (15): 4639–45. doi:10.1093/nar/gkg483. PMC 169873. PMID 12888525.
• Yamamoto Y, Fujimoto Y, Arai R, Fujie M, Usami S, Yamada T (2003). "Retrotransposon-mediated restoration of Chlorella telomeres: accumulation of Zepp retrotransposons at termini of newly formed minichromosomes". Nucleic Acids Res. 31 (15): 4646–53. doi:10.1093/nar/gkg490. PMC 169880. PMID 12888526.
• Flanders JA, Feng Q, Bagrodia S, Laux MT, Singavarapu A, Cerione RA (2003). "The Cbl proteins are binding partners for the Cool/Pix family of p21-activated kinase-binding proteins". FEBS Lett. 550 (1–3): 119–23. doi:10.1016/S0014-5793(03)00853-6. PMID 12935897.
• Shin EY, Woo KN, Lee CS, Koo SH, Kim YG, Kim WJ, Bae CD, Chang SI, Kim EG (2004). "Basic fibroblast growth factor stimulates activation of Rac1 through a p85 betaPIX phosphorylation-dependent pathway". J. Biol. Chem. 279 (3): 1994–2004. doi:10.1074/jbc.M307330200. PMID 14557270.
• Lim CS, Kim SH, Jung JG, Kim JK, Song WK (2004). "Regulation of SPIN90 phosphorylation and interaction with Nck by ERK and cell adhesion". J. Biol. Chem. 278 (52): 52116–23. doi:10.1074/jbc.M310974200. PMID 14559906.

External links

• ARHGEF7 Info with links in the Cell Migration Gateway