BOSC23

BOSC 23 is a human kidney cell line developed by Warren Pear in David Baltimore's lab [1] and is derived from the 293T cell line.[2] The main use of BOSC 23 is the production of recombinant retroviruses; it stably expresses Moloney murine leukemia virus proteins and when transiently transfected with recombinant retroviral vector DNA will produce high titers of infectious retroviral particles. The cell line does not produce detectable replication-competent virus, an important safety feature.

BOSC 23 carries neomycin/G418 resistance derived from its parental line 293T, and also hygromycin and mycophenolic acid (gpt) resistance. It should be maintained under gpt selection.

This cell line is a model for cancer research which doesn't express activated Src protein.[3]

References

  1. ↑ http://www.bio.net/bionet/mm/methods/2000-December/086646.html
  2. ↑ Pear WS, Nolan GP, Scott ML, Baltimore D (15 Sep 1993). "Production of high-titer helper-free retroviruses by transient transfection". Proc. Natl. Acad. Sci. USA. 90 (18): 8392–8396. PMC 47362Freely accessible. PMID 7690960.
  3. ↑ Goh YM, Cinghu S, Hong ETH et al. Src kinase phosphorylates RUNX3 at tyrosine residues and localizes the protein in the cytoplasm. The Journal of Biochemistry vol.285, no.13, pp.10122-10129, March 2010

External links

This article is issued from Wikipedia - version of the 8/13/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.