Calitoxin

Calitoxin

Identifiers
CAS Number	118354-83-9
Abbreviations	CLX
Properties
Chemical formula	C203H305N55O72S7
Molar mass	4,892.41 g·mol^−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Calitoxin-1
Identifiers
Organism	Calliactis parasitica
Symbol	CLX-1
UniProt	P14531

Calitoxin-2
Identifiers
Organism	Calliactis parasitica
Symbol	CLX-2
UniProt	P49127

Identifiers
Symbol	Toxin_4
Pfam	PF00706
InterPro	IPR000693
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Calitoxin, also known as CLX, is a sea anemone neurotoxin produced by the sea anemone Calliactis parasitica. It targets crabs and octopuses, among other invertebrates. Two isoforms (CLX-1 and CLX-2) have been identified, both of which are formed from precursors stored in the stinging cells of the anemone. Once the toxin is activated and released, it causes paralysis by increasing neurotransmitter release at invertebrate neuromuscular junctions. Along with several other toxins derived from anemones, CLX is useful in ion channel research. Certain structural aspects of calitoxin are dissimilar from sea anemone toxins that also target the sodium ion channels. Other toxins resembling calitoxin function in completely different ways.

Source and discovery

Calitoxin is a highly potent neurotoxin produced by the sea anemone Calliactis parasitica, which is stored in the nematocysts of stinging cells (cnidocytes).^[1] This sea anemone is a species from the Hormathiidae family and is present along the European coasts of the Atlantic Ocean and in the Mediterranean Sea. ^[2] The name calitoxin is derived from the organism from which the toxin was isolated. The toxin was isolated by a team of researchers in Naples, Italy from animals collected in the Bay of Naples. The team isolated the polypeptide through a series of centrifugations until the supernatant had lost toxic activity. The resulting pellet was purified using the techniques liquid chromatography, gel filtration, and chromatofocusing.^[3] The team then sequenced the purified polypeptide chain. They also published details on the toxin's effects in vitro on crustacean tissue preparations, including nerve and muscle. Their findings were published in the journal Biochemistry in 1989.^[2]

Structure and chemistry

The formula for calitoxin is C₂₀₃H₃₀₅N₅₅O₇₂S₇. It has a molecular mass of 4886 Daltons and an isoelectric point at pH 5.4.^[1] The amino acid sequence is markedly dissimilar from other known sea anemones toxins. There are two known genes coding for two highly homologous calitoxins—CLX-1 and CLX-2. Both originate from a precursor peptide of 79 amino acids where the C-terminus determines whether it will be the mature CLX-1 or CLX-2. The activated toxins consist of 46 amino acids with three disulfide bonds.^[4] Researchers suspect that the toxins are stored as precursors in cnidocytes. Under the effects of some triggering stimulus, the precursor is modified and released in the active form. The patterning of cleavage sites targeted during maturation of the peptide suggest that the active quaternary structure might be a tetrapeptide.^[3]

Isoform	Sequence	Location disulfide bridges
CLX-1 precursor	MKTQVLALFV LCVLFCLAES RTTLNKRNDI EKRIECKCEG DAPDLSHMTG TVYFSCKGGD GSWSKCNTYT AVADCCHQA	36 – 75, 38 – 66, 56 – 76[5]
CLX-2 precursor	MKTQVLAVFV LCVLFCLAES RTTLNKRIDI AKRIECKCKG DAPDLSHMTG TVYFSCKGGD GSWSKCNTYT AVADCCHQA	36 – 75, 38 – 66, 56 – 76[6]

Calitoxin and other sea anemone toxins are used in studying ion channels, with potential applications in biomedical and physiology research.^[7][3] In the mature CLX, one base-pair substitution is responsible for a single glutamic acid to lysine replacement in the coding region of CLX-2, leading to the difference between the two isoforms. The structural organization of these two genes show a high degree of homology. This suggests that the two different peptides have the same biological function. This cannot yet be confirmed because only CLX-1 has been isolated from C. parasitica.^[1] Calitoxin has a very different sequence from another sodium channel binding sea anemone toxin, ATX II, which is produced by the distantly related Anemonia sulcata.^[8] A better understanding of these differences might offer insights about the function of particular amino acid residues.^[1] Despite markedly dissimilar gene sequences, CLX-1 affects crustacean axon potentials similar to two other classes of anemone toxins. Alternatively, certain aspects of the structure of the CLX genes are found in scorpion toxins as well as other sea anemone toxins that block potassium channels.^[9]

Target and activity

Calitoxin causes massive neurotransmitter release from the nerve terminals of the neuromuscular junction, which in turn causes a strong muscle contraction and even paralysis. The exact target of calitoxin has not yet been clarified; since it has a similar action on the neuromuscular junction as Anemonia sulcata toxins, calitoxin may slow down the inactivation of voltage-gated sodium channels in motor neurons. Calitoxin has been tested for activity on the crab Carcinus mediterraneus. Purified toxin was injected into the hemocoel of the crab. The minimum dose of 0.2 µg of toxin triggered muscle contractions in the crab, causing paralysis within 1 minute. The median lethal dose (LD₅₀) is unknown.^[2]

Function in nature

Calliactis parasitica with a symbiotic hermit crab

Sea anemones produce toxins, such as calitoxin, in their stinging cells (cnidocytes). These cells contain organelles called nematocysts. When triggered, an envenomation response occurs. This can result in injury to target organisms, including capture of prey, defense against predatory organisms, or against aggressors from within their own species.^[4] In its natural setting, C. parasitica can establish a mutualistic relationship with the hermit crab Pagurus bernhardus. The sea anemone identifies shells inhabited by the hermit crab and attaches. C. parasitica provides protection for the hermit crab, by stinging or intimidating potential predators. Octopuses will avoid shells bearing C. parasitica.^[10] In return for the protection, the sea anemone gains an advantage in accessing a broader distribution of food sources, as the crab moves across the ocean floor.^[11]

References

1. Spagnuolo, Antonietta; Zanetti, Laura; Cariello, Lucio; Piccoli, Renata. "Isolation and characterization of two genes encoding calitoxins, neurotoxic peptides from Calliactis parasitica (Cnidaria)". Gene. 138 (1-2): 187–191. doi:10.1016/0378-1119(94)90805-2.
2. Cariello, L; de Santis, A; Fiore, F; Piccoli, R; Spagnuolo, A; Zanetti, L; Parente, A (21 Mar 1989). "Calitoxin, a neurotoxic peptide from the sea anemone Calliactis parasitica: amino acid sequence and electrophysiological properties". Biochemistry. 28 (6): 2484–9. doi:10.1021/bi00432a020. PMID 2567180.
3. Rappuoli, Rino; Montecucco, Cesare (29 May 1997). Guidebook to Protein Toxins and Their Use in Cell Biology. Oxford University Press, UK. pp. 139–. ISBN 978-0-19-154728-7.
4. Kastin, edited by Abba J. (2006). Handbook of Biologically Active Peptides. Amsterdam: Academic Press. pp. 363–364. ISBN 0-12-369442-6.
5. "Calitoxin-1". UniProt.
6. "Calitoxin-2". UniProt.
7. Nagai, Hiroshi (2012). "Special Issue "Sea Anemone Toxins"". Marine Drugs.
8. Q. Ashton Acton (2013). Neurologic Manifestations—Advances in Research and Treatment. ScholarlyEditions. p. 60. ISBN 9781481678049.
9. Moran, Yehu; Gordon, Dalia; Gurevitz, Michael (December 2009). "Sea anemone toxins affecting voltage-gated sodium channels – molecular and evolutionary features". Toxicon. 54 (8): 1089–1101. doi:10.1016/j.toxicon.2009.02.028.
10. Roger T. Hanlon & John B. Messenger (1998). "Learning and the development of behaviour". Cephalopod Behaviour. Cambridge University Press. pp. 132–148. ISBN 978-0-521-64583-6.
11. John Fish & Susan Fish (2011). "Calliactis parasitica (Couch)". A Student's Guide to the Seashore (3rd ed.). Cambridge University Press. p. 96. ISBN 978-0-521-72059-5.