Glycoside hydrolase family 25
| Glycosyl hydrolases family 25 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 crystal structure of the bacterial lysozyme from streptomyces coelicolor at 1.65 a resolution | |||||||||
| Identifiers | |||||||||
| Symbol | Glyco_hydro_25 | ||||||||
| Pfam | PF01183 | ||||||||
| Pfam clan | CL0058 | ||||||||
| InterPro | IPR002053 | ||||||||
| PROSITE | PDOC00737 | ||||||||
| SCOP | 1jfx | ||||||||
| SUPERFAMILY | 1jfx | ||||||||
| CAZy | GH25 | ||||||||
| |||||||||
In molecular biology, glycoside hydrolase family 25 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[5]
Glycoside hydrolase family 25 CAZY GH_25 comprises enzymes with only one known activity; lysozyme (EC 3.2.1.17). It has been shown[6][7] that a number of cell-wall lytic enzymes are evolutionary related and can be classified into a single family. Two residues, an aspartate and a glutamate, have been shown[8] to be important for the catalytic activity of the Charalopsis enzyme. These residues as well as some others in their vicinity are conserved in all proteins from this family.
References
- ↑ Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477
. PMID 7624375. - ↑ Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ↑ Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
- ↑ Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
- ↑ CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
- ↑ Henrissat B (December 1991). "A classification of glycosyl hydrolases based on amino acid sequence similarities". Biochem. J. 280 (2): 309–16. doi:10.1042/bj2800309. PMC 1130547. PMID 1747104.
- ↑ Croux C, Garcia JL (1991). "Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824: comparison with other lytic enzymes". Gene. 104 (1): 25–31. doi:10.1016/0378-1119(91)90460-S. PMID 1916274.
- ↑ Fouche PB, Hash JH (1978). "The N,O-diacetylmuramidase of Chalaropsis species. Identification of aspartyl and glutamyl residues in the active site". J. Biol. Chem. 253 (19): 6787–6793. PMID 567645.
This article incorporates text from the public domain Pfam and InterPro IPR002053