Glycoside hydrolase family 44
| Glycoside hydrolase family 44 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Glyco_hydro_44 | ||||||||
| Pfam | PF12891 | ||||||||
| Pfam clan | CL0058 | ||||||||
| CAZy | GH44 | ||||||||
| |||||||||
In molecular biology, glycoside hydrolase family 44 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[5]
Glycoside hydrolase family 44 CAZY GH_44, formerly known as cellulase family J, includes enzymes with endoglucanase EC 3.2.1.4 and xyloglucanase EC 3.2.1.151 activities. Its structure consists of a TIM-like barrel domain and a beta-sandwich domain, and the active site residues are two glutamic acid residues.[6]
References
- ↑ Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477
. PMID 7624375. - ↑ Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ↑ Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
- ↑ Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
- ↑ CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
- ↑ Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, et al. (2007). "Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum.". J Biol Chem. 282 (49): 35703–11. doi:10.1074/jbc.M706835200. PMID 17905739.