Hexadimethrine bromide

Hexadimethrine bromide
Names
IUPAC name
1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide
Other names
Polybrene
Identifiers
28728-55-4 YesY
ECHA InfoCard 100.209.698
Properties
(C13H30Br2N2)n, linear form
Molar mass variable
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Hexadimethrine bromide (commercial brand name Polybrene) is a cationic polymer used to increase the efficiency of transduction of certain cells with virus in cell culture.[1] Hexadimethrine bromide acts by neutralizing the charge repulsion between virions and sialic acid on the cell surface.[2] Use of Polybrene can improve transduction efficiency 100-1000 fold[3] although it can be toxic to some cell types. Polybrene in combination with DMSO shock is used to transfect some cell types such as NINH-3T3 and CHO[3] . It has other uses, including a role in protein sequencing.

Historically, hexadimethrine bromide was sometimes used to reverse heparin anticoagulation during open-heart surgery. Its use has been supplanted by protamine sulfate.

References

Polybrene® is a registered trademark of Abbott Laboratories Corp.

  1. Howard e. Davis; Jeffery R. Morgan; Martin L. Yarmush (2002). "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on the target cell membranes.". Biophysical Chemistry. 97 (2): 159–172. doi:10.1016/S0301-4622(02)00057-1.
  2. Howard E. Davis; Matthew Rosinski; Jeffrey R. Morgan; Martin L. Yarmush; et al. (2004). "Charged Polymers Modulate Retrovirus Transduction via Membrane Charge Neutralization and Virus Aggregation.". Biophysical Journal. 86 (2): 1234–42. doi:10.1016/S0006-3495(04)74197-1. PMC 1303915Freely accessible. PMID 14747357.
  3. 1 2 "Polybrene Infection / Transfection Reagent | TR-1003-G". www.emdmillipore.com. Retrieved 2016-12-02.


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