HpaII

HpaII (IntEnz: EC 3.1.21.4) is a restriction enzyme obtained from the microorganism called Haemophilus parainfluenzae. It is a DNA restriction enzyme, therefore it has the ability to cut the DNA from certain region as demonstrated below.[1] It has the ability to produce cohesive ends, which are rather useful in constructing plasmids.

Recognition sequence Cut results
5' CCGG
3' GGCC
 
5' C  CGG
3' GGC  C

HpaII enzyme can cleave a DNA only when it is not protected by methylation.[2] This feature is exploited for determination of the clonal origin of a mammalian female tumor through HUMARA assay.

References

  1. ↑ InterPro: IPR019062
  2. ↑ Waalwijk, C; Flavell, R A. "MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites". 5: 3231. PMC 342244Freely accessible.
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