KAHRP

KAHRP (Plasmodium falciparum knob-associated histidine-rich protein) is a protein expressed in erythrocytes infected by Plasmodium falciparum. KAHRP is a major component of knobs, feature found on Plasmodium falciparum infected erythrocytes.

It has been suggested that KAHRP may play a role in trafficking or docking PfEMP1, major malarial cytoadherence protein to the erythrocyte membrane;[1] however, these findings were disputed by recent NMR and fluorescence anisotropy studies showing no interaction between PfEMP1 and KAHRP.[2]

Instead, KAHRP was shown to interact with Ankyrin, more precisely the D3 subunit of the Membrane-binding domain of Ankyrin type 1.[3] This interaction was suggested via SPR, ELISA, and Pulldown studies, however, it has not been confirmed by NMR, ITC, crystallography, or fluorescence anisotropy.

References

  1. Alexander G. Maier, Brian M. Cooke, Alan F. Cowman & Leann Tilley (May 2009). "Malaria parasite proteins that remodel the host erythrocyte : Abstract". Nature Reviews Microbiology. 7 (5): 341–354. doi:10.1038/nrmicro2110. Retrieved 2014-03-15.
  2. Christina Mayer; Leanne Slater; Michele C. Erat; Robert Konrat; Ioannis Vakonakis (March 2, 2012). "Structural Analysis of the Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) Intracellular Domain Reveals a Conserved Interaction Epitope". J Biol Chem. 287(10) (10): 7182–7189. doi:10.1074/jbc.M111.330779. PMC 3293552Freely accessible. PMID 22249178.
  3. Weng H1, Guo X, Papoin J, Wang J, Coppel R, Mohandas N, An X. (January 24, 2014). "Interaction of Plasmodium falciparum knob-associated histidine-rich protein (KAHRP) with erythrocyte ankyrin R is required for its attachment to the erythrocyte membrane". Biochim Biophys Acta. 1838 (1 Pt B): 185–192. doi:10.1016/j.bbamem.2013.09.014. PMC 4403245Freely accessible. PMID 24090929.


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