PABPC1

PABPC1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1CVJ, 1G9L, 1JGN, 1JH4, 2K8G, 2RQG, 2RQH, 2X04, 3KTP, 3KTR, 3KUI, 3KUJ, 3KUR, 3KUS, 3KUT, 3PKN, 3PTH, 4F02, 4F25, 4F26, 5DX1, 5DXA, 5DX8, 2D9P
Identifiers
Aliases	PABPC1, PAB1, PABP, PABP1, PABPC2, PABPL1, poly(A) binding protein cytoplasmic 1
External IDs	MGI: 1349722 HomoloGene: 37638 GeneCards: PABPC1
Gene ontology Molecular function • nucleotide binding • protein binding • poly(A) RNA binding • nucleic acid binding • protein C-terminus binding • translation activator activity • poly(U) RNA binding • RNA binding • poly(A) binding • mRNA 3'-UTR binding Cellular component • intracellular ribonucleoprotein complex • focal adhesion • catalytic step 2 spliceosome • cytoplasmic stress granule • cytoplasm • cytoplasmic ribonucleoprotein granule • spliceosomal complex • membrane • cytosol • extracellular exosome • nucleus Biological process • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay • mRNA stabilization • mRNA splicing, via spliceosome • regulation of mRNA stability • RNA splicing • mRNA polyadenylation • mRNA processing • nuclear-transcribed mRNA poly(A) tail shortening • positive regulation of viral genome replication • translational initiation • negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay • positive regulation of translation • gene silencing by RNA • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	26986	18458
Ensembl	ENSG00000070756	ENSMUSG00000022283
UniProt	P11940	P29341
RefSeq (mRNA)	NM_002568	NM_008774
RefSeq (protein)	NP_002559.2	NP_032800.2
Location (UCSC)	Chr 8: 100.69 – 100.72 Mb	Chr 15: 36.6 – 36.61 Mb
PubMed search	[1]	[2]
Wikidata

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Polyadenylate-binding protein 1 is a protein that in humans is encoded by the PABPC1 gene.^[3]

Function

The poly(A)-binding protein (PAB or PABP), which is found complexed to the 3' poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.[supplied by OMIM]^[4]

Interactions

PABPC1 has been shown to interact with:

• ANAPC5,^[5]
• CNOT7,^[6]
• EIF4G3,^[7]
• EIF4G1,^[7]
• GSPT2,^[8]
• PAIP1,^[9][10] and
• PAIP2.^[11]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Grange T, de Sa CM, Oddos J, Pictet R (Aug 1987). "Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif". Nucleic Acids Res. 15 (12): 4771–87. doi:10.1093/nar/15.12.4771. PMC 305917. PMID 2885805.
4. "Entrez Gene: PABPC1 poly(A) binding protein, cytoplasmic 1".
5. Koloteva-Levine N, Pinchasi D, Pereman I, Zur A, Brandeis M, Elroy-Stein O (May 2004). "The Apc5 subunit of the anaphase-promoting complex/cyclosome interacts with poly(A) binding protein and represses internal ribosome entry site-mediated translation". Mol. Cell. Biol. 24 (9): 3577–87. doi:10.1128/mcb.24.9.3577-3587.2004. PMC 387753. PMID 15082755.
6. Funakoshi Y, Doi Y, Hosoda N, Uchida N, Osawa M, Shimada I, Tsujimoto M, Suzuki T, Katada T, Hoshino S (Dec 2007). "Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases". Genes Dev. 21 (23): 3135–48. doi:10.1101/gad.1597707. PMC 2081979. PMID 18056425.
7. Imataka H, Gradi A, Sonenberg N (Dec 1998). "A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation". EMBO J. 17 (24): 7480–9. doi:10.1093/emboj/17.24.7480. PMC 1171091. PMID 9857202.
8. Hoshino S, Imai M, Kobayashi T, Uchida N, Katada T (Jun 1999). "The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein". J. Biol. Chem. 274 (24): 16677–80. doi:10.1074/jbc.274.24.16677. PMID 10358005.
9. Roy G, De Crescenzo G, Khaleghpour K, Kahvejian A, O'Connor-McCourt M, Sonenberg N (Jun 2002). "Paip1 interacts with poly(A) binding protein through two independent binding motifs". Mol. Cell. Biol. 22 (11): 3769–82. doi:10.1128/mcb.22.11.3769-3782.2002. PMC 133836. PMID 11997512.
10. Craig AW, Haghighat A, Yu AT, Sonenberg N (Apr 1998). "Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation". Nature. 392 (6675): 520–3. doi:10.1038/33198. PMID 9548260.
11. Khaleghpour K, Kahvejian A, De Crescenzo G, Roy G, Svitkin YV, Imataka H, O'Connor-McCourt M, Sonenberg N (Aug 2001). "Dual interactions of the translational repressor Paip2 with poly(A) binding protein". Mol. Cell. Biol. 21 (15): 5200–13. doi:10.1128/MCB.21.15.5200-5213.2001. PMC 87244. PMID 11438674.

Further reading

• Görlach M, Burd CG, Dreyfuss G (1994). "The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity". Exp. Cell Res. 211 (2): 400–7. doi:10.1006/excr.1994.1104. PMID 7908267.
• Campbell LH, Borg KT, Haines JK, Moon RT, Schoenberg DR, Arrigo SJ (1994). "Human immunodeficiency virus type 1 Rev is required in vivo for binding of poly(A)-binding protein to Rev-dependent RNAs". J. Virol. 68 (9): 5433–8. PMC 236943. PMID 8057425.
• Huang Y, Carmichael GG (1996). "Role of polyadenylation in nucleocytoplasmic transport of mRNA". Mol. Cell. Biol. 16 (4): 1534–42. PMC 231138. PMID 8657127.
• Craig AW, Haghighat A, Yu AT, Sonenberg N (1998). "Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation". Nature. 392 (6675): 520–3. doi:10.1038/33198. PMID 9548260.
• Afonina E, Stauber R, Pavlakis GN (1998). "The human poly(A)-binding protein 1 shuttles between the nucleus and the cytoplasm". J. Biol. Chem. 273 (21): 13015–21. doi:10.1074/jbc.273.21.13015. PMID 9582337.
• Imataka H, Gradi A, Sonenberg N (1999). "A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation". EMBO J. 17 (24): 7480–9. doi:10.1093/emboj/17.24.7480. PMC 1171091. PMID 9857202.
• Hornstein E, Git A, Braunstein I, Avni D, Meyuhas O (1999). "The expression of poly(A)-binding protein gene is translationally regulated in a growth-dependent fashion through a 5'-terminal oligopyrimidine tract motif". J. Biol. Chem. 274 (3): 1708–14. doi:10.1074/jbc.274.3.1708. PMID 9880551.
• Laroia G, Cuesta R, Brewer G, Schneider RJ (1999). "Control of mRNA decay by heat shock-ubiquitin-proteasome pathway". Science. 284 (5413): 499–502. doi:10.1126/science.284.5413.499. PMID 10205060.
• Hoshino S, Imai M, Kobayashi T, Uchida N, Katada T (1999). "The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein". J. Biol. Chem. 274 (24): 16677–80. doi:10.1074/jbc.274.24.16677. PMID 10358005.
• Deo RC, Bonanno JB, Sonenberg N, Burley SK (1999). "Recognition of polyadenylate RNA by the poly(A)-binding protein". Cell. 98 (6): 835–45. doi:10.1016/S0092-8674(00)81517-2. PMID 10499800.
• Féral C, Mattéi MG, Pawlak A, Guellaën G (1999). "Chromosomal localization of three human poly(A)-binding protein genes and four related pseudogenes". Hum. Genet. 105 (4): 347–53. doi:10.1007/s004390051113. PMC 1865476. PMID 10543404.
• Grosset C, Chen CY, Xu N, Sonenberg N, Jacquemin-Sablon H, Shyu AB (2000). "A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex". Cell. 103 (1): 29–40. doi:10.1016/S0092-8674(00)00102-1. PMID 11051545.
• Khaleghpour K, Svitkin YV, Craig AW, DeMaria CT, Deo RC, Burley SK, Sonenberg N (2001). "Translational repression by a novel partner of human poly(A) binding protein, Paip2". Mol. Cell. 7 (1): 205–16. doi:10.1016/S1097-2765(01)00168-X. PMID 11172725.
• Bushell M, Wood W, Carpenter G, Pain VM, Morley SJ, Clemens MJ (2001). "Disruption of the interaction of mammalian protein synthesis eukaryotic initiation factor 4B with the poly(A)-binding protein by caspase- and viral protease-mediated cleavages". J. Biol. Chem. 276 (26): 23922–8. doi:10.1074/jbc.M100384200. PMID 11274152.
• Féral C, Guellaën G, Pawlak A (2002). "Human testis expresses a specific poly(A)-binding protein". Nucleic Acids Res. 29 (9): 1872–83. doi:10.1093/nar/29.9.1872. PMC 37253. PMID 11328870.
• Khaleghpour K, Kahvejian A, De Crescenzo G, Roy G, Svitkin YV, Imataka H, O'Connor-McCourt M, Sonenberg N (2001). "Dual Interactions of the Translational Repressor Paip2 with Poly(A) Binding Protein". Mol. Cell. Biol. 21 (15): 5200–13. doi:10.1128/MCB.21.15.5200-5213.2001. PMC 87244. PMID 11438674.
• Woods AJ, Roberts MS, Choudhary J, Barry ST, Mazaki Y, Sabe H, Morley SJ, Critchley DR, Norman JC (2002). "Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells". J. Biol. Chem. 277 (8): 6428–37. doi:10.1074/jbc.M109446200. PMID 11704675.
• Lee J, Bedford MT (2002). "PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays". EMBO Rep. 3 (3): 268–73. doi:10.1093/embo-reports/kvf052. PMC 1084016. PMID 11850402.
• Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.