PrfA thermoregulator UTR

PrfA thermoregulator UTR
Predicted secondary structure and sequence conservation of PrfA
Identifiers
Symbol PrfA
Rfam RF00038
Other data
RNA type Cis-reg; thermoregulator
Domain(s) Bacteria
SO 0000233

The PrfA thermoregulator UTR is an RNA thermometer found in the 5' UTR of the prfA gene.[1] In Listeria monocytogenes, virulence genes are maximally expressed at 37 °C (human body temperature) but are almost silent at 30 °C.[2] The genes are controlled by PrfA, a transcriptional activator whose expression is thermoregulated.[3] It has been shown that the untranslated mRNA (UTR) preceding prfA, forms a secondary structure, which masks the ribosome binding region.[1] It is thought that at 37 °C, the hairpin structure 'melts' and the SD sequence is unmasked.[1]

References

  1. 1 2 3 Johansson J, Mandin P, Renzoni A, Chiaruttini C, Springer M, Cossart P (2002). "An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes". Cell. 110 (5): 551–61. doi:10.1016/S0092-8674(02)00905-4. PMID 12230973.
  2. Freitag, NE; Port, GC; Miner, MD (September 2009). "Listeria monocytogenes - from saprophyte to intracellular pathogen.". Nature reviews. Microbiology. 7 (9): 623–8. doi:10.1038/nrmicro2171. PMC 2813567Freely accessible. PMID 19648949.
  3. Digel, I; Kayser, P; Artmann, GM (2008). "Molecular processes in biological thermosensation.". Journal of biophysics (Hindawi Publishing Corporation : Online). 2008: 602870. doi:10.1155/2008/602870. PMC 2814129Freely accessible. PMID 20130806.

External links


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