RNA spike-in

An RNA spike-in is an RNA transcript used to calibrate measurements in a DNA microarray experiment. Each spike-in is designed to hybridize with a specific control probe on the target array. Manufacturers of commercially available microarrays typically offer companion RNA spike-ins "kits".[1][2][3][4]

Known amounts of RNA spike-ins are mixed with the experiment sample during preparation. Subsequently the measured degree of hybridization between the spike-ins and the control probes is used to normalize the hybridization measurements of the sample RNA.

See also

References

  1. Yang IV (2006). "Use of external controls in microarray experiments.". Methods Enzymol. 411: 50–63. doi:10.1016/S0076-6879(06)11004-6. PMID 16939785.
  2. Fardin P, Moretti S, Biasotti B, Ricciardi A, Bonassi S, Varesio L (2007). "Normalization of low-density microarray using external spike-in controls: analysis of macrophage cell lines expression profile.". BMC Genomics. 8: 17. doi:10.1186/1471-2164-8-17. PMC 1797020Freely accessible. PMID 17229315.
  3. Wilkes T, Laux H, Foy CA (2007). "Microarray data quality - review of current developments.". OMICS. 11 (1): 1–13. doi:10.1089/omi.2006.0001. PMID 17411392.
  4. Schuster EF, Blanc E, Partridge L, Thornton JM (2007). "Estimation and correction of non-specific binding in a large-scale spike-in experiment.". Genome Biol. 8 (6): R126. doi:10.1186/gb-2007-8-6-r126. PMC 2394775Freely accessible. PMID 17594493.


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