Radiobinding assay

A radiobinding assay is a method of detecting and quantifying antibodies targeted towards a specific antigen. As such, it can be seen as the inverse of radioimmunoassay, which quantifies an antigen by use of corresponding antibodies.

Technique

The corresponding antigen is radiolabeled and mixed with the fluid that may contain the antibody, such as blood serum from a person. Presence of antibodies cause precipitation of antibody-antigen complexes that can be collected by centrifugation into pellets. The amount of antibody is proportional to the radioactivity of the pellet, as determined by gamma counting.^[1]

Uses

It is used to detect most autoantibodies seen in latent autoimmune diabetes.^[2]

References

↑ Anti-dsDNA [I-125] Radiobinding Assay Kit At PerkinElmer Life Sciences, Inc. Retrieved Jan 2011
↑ Knip, M.; Veijola, R.; Virtanen, S. M.; Hyoty, H.; Vaarala, O.; Akerblom, H. K. (2005). "Environmental Triggers and Determinants of Type 1 Diabetes". Diabetes. 54: S125–S136. doi:10.2337/diabetes.54.suppl_2.S125. PMID 16306330.

Medical tests: Immunologic techniques and tests (CPT 86000–86849)

Immunologic techniques
and tests

serology/
diagnostic immunology

Immunoprecipitation	Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion Immunoelectrophoresis Counterimmunoelectrophoresis

Immunoassay	ELISA ELISPOT Enzyme Multiplied Immunoassay Technique RAST test Radioimmunoassay Radiobinding assay Immunofluorescence

Agglutination	Hemagglutination/Hemagglutinin Coombs test Latex fixation test

Other	Nephelometry Complement fixation test Immunocytochemistry Immunohistochemistry Direct fluorescent antibody Epitope mapping Skin allergy test Patch test

Inflammation

CBC
- lymphocyte count

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