Ribonuclease T

Ribonuclease T

A ribonuclease T dimer in complex with DNA (orange), from PDB ID 3NH1.^[1]

Identifiers

Symbol

rnt

Available protein structures:
Pfam	structures
PDB	RCSB PDB; PDBe; PDBj
PDBsum	structure summary

Ribonuclease T (RNase T, exonuclease T, exo T) is a ribonuclease enzyme involved in the maturation of transfer RNA and ribosomal RNA in bacteria,^[2] as well as in DNA repair pathways.^[3] It is a member of the DnaQ family of exonucleases and non-processively acts on the 3' end of single-stranded nucleic acids. RNase T is capable of cleaving both DNA and RNA, with extreme sequence specificity discriminating against cytosine at the 3' end of the substrate.^[1]^[2]

References

1 2 Hsiao, Y.-Y.; Duh, Y.; Chen, Y.-P.; Wang, Y.-T.; Yuan, H. S. (2012). "How an exonuclease decides where to stop in trimming of nucleic acids: Crystal structures of RNase T-product complexes". Nucleic Acids Research. 40 (16): 8144–54. doi:10.1093/nar/gks548. PMID 22718982.
1 2 Zuo, Y.; Deutscher, M. P. (2002). "The Physiological Role of RNase T Can Be Explained by Its Unusual Substrate Specificity". Journal of Biological Chemistry. 277 (33): 29654–61. doi:10.1074/jbc.M204252200. PMID 12050169.
↑ Hsiao, Yu-Yuan; Fang, Woei-Horng; Lee, Chia-Chia; Chen, Yi-Ping; Yuan, Hanna S. (2014). "Structural Insights into DNA Repair by RNase T—An Exonuclease Processing 3′ End of Structured DNA in Repair Pathways". PLoS Biology. 12 (3): e1001803. doi:10.1371/journal.pbio.1001803. PMC 3942315. PMID 24594808.

3.1.1: Carboxylic
ester hydrolases

Endodeoxyribonuclease	Deoxyribonuclease I Deoxyribonuclease II Deoxyribonuclease IV Restriction enzyme UvrABC endonuclease

Endoribonuclease	RNase III RNase H 1 2A 2B 2C RNase P RNase A 1 2 3 4/5 RNase T1 RNA-induced silencing complex

either deoxy- or ribo-	Aspergillus nuclease S1 Micrococcal nuclease

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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