MDFI

MDFI
Identifiers
Aliases	MDFI, MyoD family inhibitor, I-MF, I-mfa
External IDs	MGI: 107687 HomoloGene: 21150 GeneCards: MDFI
Gene ontology Molecular function • protein binding • identical protein binding • transcription factor binding Cellular component • cytoplasm • nucleus Biological process • cytoplasmic sequestering of transcription factor • embryonic skeletal system morphogenesis • multicellular organism development • negative regulation of DNA binding • negative regulation of Wnt signaling pathway • trophoblast giant cell differentiation • cell differentiation • regulation of Wnt signaling pathway • negative regulation of transcription from RNA polymerase II promoter • activation of JUN kinase activity • embryo development • dorsal/ventral axis specification • negative regulation of transcription, DNA-templated Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4188	17240
Ensembl	ENSG00000112559	ENSMUSG00000032717
UniProt	Q99750	P70331
RefSeq (mRNA)	NM_001300804 NM_001300805 NM_001300806 NM_005586	NM_001109973 NM_001276390 NM_001276391 NM_010783
RefSeq (protein)	NP_001287733.1 NP_001287734.1 NP_001287735.1 NP_005577.1	NP_001103443.1 NP_001263319.1 NP_034913.2
Location (UCSC)	Chr 6: 41.64 – 41.65 Mb	Chr 17: 47.82 – 47.83 Mb
PubMed search	[1]	[2]
Wikidata

View/Edit Human

View/Edit Mouse

MyoD family inhibitor is a protein that in humans is encoded by the MDFI gene.^[3][4][5]

Function

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.^[5]

Interactions

MDFI has been shown to interact with:

• C9orf86,^[6]
• GNAI2,^[6]
• MyoD,^[7]
• Myogenin,^[7] and
• SIX1.^[6]

References

1. "Human PubMed Reference:".
2. "Mouse PubMed Reference:".
3. Kraut N (Sep 1997). "The gene encoding I-mf (Mdfi) maps to human chromosome 6p21 and mouse chromosome 17". Mamm Genome. 8 (8): 618–9. doi:10.1007/s003359900517. PMID 9250874.
4. Wang Q, Young TM, Mathews MB, Pe'ery T (Mar 2007). "Developmental regulators containing the I-mfa domain interact with T cyclins and Tat and modulate transcription". J Mol Biol. 367 (3): 630–46. doi:10.1016/j.jmb.2007.01.020. PMC 1868487. PMID 17289077.
5. "Entrez Gene: MDFI MyoD family inhibitor".
6. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
7. Chen CM, Kraut N, Groudine M, Weintraub H (Sep 1996). "I-mf, a novel myogenic repressor, interacts with members of the MyoD family". Cell. 86 (5): 731–41. doi:10.1016/s0092-8674(00)80148-8. PMID 8797820.

Further reading

• Chen CM, Kraut N, Groudine M, Weintraub H (1996). "I-mf, a novel myogenic repressor, interacts with members of the MyoD family.". Cell. 86 (5): 731–41. doi:10.1016/S0092-8674(00)80148-8. PMID 8797820.
• Kraut N, Snider L, Chen CM, Tapscott SJ, Groudine M (1999). "Requirement of the mouse I-mfa gene for placental development and skeletal patterning.". EMBO J. 17 (21): 6276–88. doi:10.1093/emboj/17.21.6276. PMC 1170953. PMID 9799236.
• Snider L, Thirlwell H, Miller JR, Moon RT, Groudine M, Tapscott SJ (2001). "Inhibition of Tcf3 binding by I-mfa domain proteins.". Mol. Cell. Biol. 21 (5): 1866–73. doi:10.1128/MCB.21.5.1866-1873.2001. PMC 86756. PMID 11238923.
• Ma R, Rundle D, Jacks J, Koch M, Downs T, Tsiokas L (2004). "Inhibitor of myogenic family, a novel suppressor of store-operated currents through an interaction with TRPC1.". J. Biol. Chem. 278 (52): 52763–72. doi:10.1074/jbc.M309610200. PMID 14530267.
• Mizugishi K, Hatayama M, Tohmonda T, Ogawa M, Inoue T, Mikoshiba K, Aruga J (2004). "Myogenic repressor I-mfa interferes with the function of Zic family proteins.". Biochem. Biophys. Res. Commun. 320 (1): 233–40. doi:10.1016/j.bbrc.2004.05.158. PMID 15207726.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Lim J, Hao T, Shaw C, Patel AJ, Szabó G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabási AL, Vidal M, Zoghbi HY (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.