RRNA small subunit pseudouridine methyltransferase Nep1

RRNA small subunit pseudouridine methyltransferase Nep1 (EC 2.1.1.260, Nep1, nucleolar essential protein 1) is an enzyme with systematic name S-adenosyl-L-methionine:18S rRNA (pseudouridine¹¹⁹¹-N¹)-methyltransferase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + pseudouridine¹¹⁹¹ in yeast 18S rRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + N¹-methylpseudouridine¹¹⁹¹ in yeast 18S rRNA

This enzyme recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure.

References

↑ Taylor, A.B.; Meyer, B.; Leal, B.Z.; Kötter, P.; Schirf, V.; Demeler, B.; Hart, P.J.; Entian, K.D.; Wöhnert, J. (2008). "The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site". Nucleic Acids Res. 36: 1542–1554. doi:10.1093/nar/gkm1172. PMID 18208838.
↑ Wurm, J.P.; Meyer, B.; Bahr, U.; Held, M.; Frolow, O.; Kötter, P.; Engels, J.W.; Heckel, A.; Karas, M.; Entian, K.D.; Wöhnert, J. (2010). "The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N¹-specific methyltransferase". Nucleic Acids Res. 38: 2387–2398. doi:10.1093/nar/gkp1189. PMID 20047967.
↑ Meyer, B.; Wurm, J.P.; Kötter, P.; Leisegang, M.S.; Schilling, V.; Buchhaupt, M.; Held, M.; Bahr, U.; Karas, M.; Heckel, A.; Bohnsack, M.T.; Wöhnert, J.; Entian, K.D. (2011). "The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ¹¹⁹¹ in yeast 18S rRNA". Nucleic Acids Res. 39: 1526–1537. doi:10.1093/nar/gkq931. PMID 20972225.

External links

RRNA small subunit pseudouridine methyltransferase Nep1 at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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