TRNA (adenine58-N1)-methyltransferase

TRNA (adenine⁵⁸-N¹)-methyltransferase (EC 2.1.1.220, tRNA m1A58 methyltransferase, tRNA (m1A58) methyltransferase, TrmI, tRNA (m1A58) Mtase, Rv2118cp, Gcd10p-Gcd14p, Trm61p-Trm6p) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (adenine⁵⁸-N¹)-methyltransferase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + adenine⁵⁸ in tRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + N¹-methyladenine⁵⁸ in tRNA

The enzyme specifically methylates adenine⁵⁸ in tRNA.

References

↑ Droogmans, L.; Roovers, M.; Bujnicki, J.M.; Tricot, C.; Hartsch, T.; Stalon, V.; Grosjean, H. (2003). "Cloning and characterization of tRNA (m¹A⁵⁸) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures". Nucleic Acids Res. 31: 2148–2156. doi:10.1093/nar/gkg314. PMID 12682365.
↑ Varshney, U.; Ramesh, V.; Madabushi, A.; Gaur, R.; Subramanya, H.S.; RajBhandary, U.L. (2004). "Mycobacterium tuberculosis Rv2118c codes for a single-component homotetrameric m¹A⁵⁸ tRNA methyltransferase". Nucleic Acids Res. 32: 1018–1027. doi:10.1093/nar/gkh207. PMID 14960715.
↑ Anderson, J.; Phan, L.; Hinnebusch, A.G. (2000). "The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae". Proc. Natl. Acad. Sci. USA. 97: 5173–5178. doi:10.1073/pnas.090102597. PMID 10779558.

External links

TRNA (adenine58-N1)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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