TRNA (guanine26-N2)-dimethyltransferase

TRNA (guanine²⁶-N²)-dimethyltransferase (EC 2.1.1.216, Trm1p, TRM1, tRNA (m22G26)dimethyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine²⁶-N²)-dimethyltransferase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + guanine²⁶ in tRNA

\rightleftharpoons

2 S-adenosyl-L-homocysteine + N²-dimethylguanine26 in tRNA

The enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions.

References

↑ Constantinesco, F.; Motorin, Y.; Grosjean, H. (1999). "Characterisation and enzymatic properties of tRNA(guanine²⁶, N²,N²-dimethyltransferase (Trm1p) from Pyrococcus furiosus". J. Mol. Biol. 291: 375–392. doi:10.1006/jmbi.1999.2976. PMID 10438627.
↑ Constantinesco, F.; Benachenhou, N.; Motorin, Y.; Grosjean, H. (1998). "The tRNA(guanine-26,N²-N²) methyltransferase (Trm1) from the hyperthermophilic archaeon Pyrococcus furiosus: cloning, sequencing of the gene and its expression in Escherichia coli". Nucleic Acids Res. 26: 3753–3761. doi:10.1093/nar/26.16.3753. PMID 9685492.
↑ Liu, J.; Liu, J.; Straby, K.B. (1998). "Point and deletion mutations eliminate one or both methyl group transfers catalysed by the yeast TRM1 encoded tRNA (m²₂G₂₆)dimethyltransferase". Nucleic Acids Res. 26: 5102–5108. doi:10.1093/nar/26.22.5102. PMID 9801306.
↑ Liu, J.; Zhou, G.Q.; Straby, K.B. (1999). "Caenorhabditis elegans ZC376.5 encodes a tRNA (m²₂G₂₆)dimethyltransferance in which ²⁴⁶arginine is important for the enzyme activity". Gene. 226: 73–81. doi:10.1016/s0378-1119(98)00550-2. PMID 10048958.

External links

TRNA (guanine26-N2)-dimethyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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