Enzyme |
PDB code |
Source |
Recognition sequence |
Cut |
Isoschizomers |
BstI |
|
Bacillus stearothermophilus 1503-4R |
5' GGATCC 3' CCTAGG |
5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' |
AccEBI, AliI, ApaCI, AsiI, BamHI, BnaI, BspAAIII, Nsp29132II |
Bst1I |
|
Bacillus stearothermophilus 1 |
5' CCWGG 3' GGWCC |
5' ---CC WGG--- 3' 3' ---GGW CC--- 5' |
AglI, BseBI, Bse17I, BstNI, CbrI, CthII, EcoRII, MvaI, SspAI, TaqXI |
Bst2I |
|
Bacillus stearothermophilus 2 |
5' CCWGG 3' GGWCC |
5' ---CC WGG--- 3' 3' ---GGW CC--- 5' |
AglI, BseBI, Bse17I, BstNI, BstOI, Bst2UI, CthII, EcoRII, MvaI |
Bst6I |
|
Bacillus stearothermophilus 6 |
5' CTCTTC 3' GAGAAG |
5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5' |
|
Bst11I |
|
Bacillus stearothermophilus 11 |
5' ACTGG 3' TGACC |
5' ---ACTGGN --- 3' 3' ---TGAC CN--- 5' |
|
Bst12I |
|
Bacillus stearothermophilus 12 |
5' GCAGC 3' CGTCG |
5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' |
AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst71I, BstV1I |
Bst19I |
|
Bacillus stearothermophilus 19 |
5' GCATC 3' CGTAG |
5' ---GCATCNNNN NN--- 3' 3' ---CGTAGNNNNNN --- 5' |
|
Bst19II |
|
Bacillus stearothermophilus 19 |
5' GATC 3' CTAG |
5' --- GATC--- 3' 3' ---CTAG --- 5' |
BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAI |
Bst28I |
|
Bacillus stearothermophilus 28 |
5' ATCGAT 3' TAGCTA |
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' |
BciBI, BdiI, Bli41I, Bsa29I, Bsp106I, BspDI, LplI, SpmI, ZhoI |
Bst38I |
|
Bacillus stearothermophilus 38 |
5' CCWGG 3' GGWCC |
5' ---CC WGG--- 3' 3' ---GGW CC--- 5' |
AorI, BseBI, Bse17I, BsiLI, BspNI, BstOI, Bst2UI, EcoRII, MvaI |
Bst40I |
|
Bacillus stearothermophilus 40 |
5' CCGG 3' GGCC |
5' ---C CGG--- 3' 3' ---GGC C--- 5' |
|
Bst71I |
|
Bacillus stearothermophilus 71 |
5' GCAGC 3' CGTCG |
5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' |
AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, BstV1I |
Bst98I |
|
Bacillus stearothermophilus C98 |
5' CTTAAG 3' GAATTC |
5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' |
|
Bst100I |
|
Bacillus stearothermophilus 100 |
5' CCWGG 3' GGWCC |
5' ---CC WGG--- 3' 3' ---GGW CC--- 5' |
AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, EcoRII, MvaI, SleI |
Bst1107I |
|
Bacillus stearothermophilus RFL1107 |
5' GTATAC 3' CATATG |
5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5' |
|
BstACI |
|
Bacillus stearothermophilus AC |
5' GRCGYC 3' CYGCRG |
5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5' |
AcyI, AhaII, AosII, AstWI, AsuIII, BsaHI, HgiI, HgiDI, Hsp92I |
BstAPI |
|
Bacillus stearothermophilus AP |
5' GCAN4NTGC 3' CGTN4NACG |
5' ---GCANNNN NTGC--- 3' 3' ---CGTN NNNNACG--- 5' |
ApaBI |
BstAUI |
|
Bacillus stearothermophilus AU |
5' TGTACA 3' ACATGT |
5' ---T GTACA--- 3' 3' ---ACATG T--- 5' |
AauI, |
BsmRI, |
Bsp1407I, BsrGI, |
Ssp4800I, SspBI |
|
BstBI |
|
Bacillus stearothermophilus B225 |
5' TTCGAA 3' AAGCTT |
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5' |
AcpI, Bim19I, Bpu14I, BspT104I, Csp45I, LspI, NspV, SspRFI |
Bst2BI |
|
Bacillus stearothermophilus 2B |
5' CACGAG 3' GTGCTC |
5' ---C ACGAG--- 3' 3' ---GTGCT C--- 5' |
|
BstBAI |
|
Bacillus stearothermophilus BA |
5' YACGTR 3' RTGCAY |
5' ---YAC GTR--- 3' 3' ---RTG CAY--- 5' |
|
BstBSI |
|
Bacillus stearothermophilus BS |
5' GTATAC 3' CATATG |
5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5' |
|
BstB7SI |
|
Bacillus stearothermophilus B7S |
5' RCCGGY 3' YGGCCR |
5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5' |
|
BstBS32I |
|
Bacillus stearothermophilus BS32 |
5' GAAGAC 3' CTTCTG |
5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' |
|
BstBZ153I |
|
Bacillus stearothermophilus BZ153 |
5' GCGCGC 3' CGCGCG |
5' ---G CGCGC--- 3' 3' ---CGCGC G--- 5' |
|
Bst4CI |
|
Bacillus stearothermophilus 4C |
5' ACNGT 3' TGNCA |
5' ---ACN GT--- 3' 3' ---TG NCA--- 5' |
|
BstC8I |
|
Bacillus stearothermophilus C8 |
5' GCNNGC 3' CGNNCG |
5' ---GCN NGC--- 3' 3' ---CGN NCG--- 5' |
|
BstD102I |
|
Bacillus stearothermophilus D102 |
5' CCGCTC 3' GGCGAG |
5' ---CCG CTC--- 3' 3' ---GGC GAG--- 5' |
AccBSI, BsrBI, Bst31NI, MbiI |
BstDEI |
|
Bacillus stearothermophilus DE |
5' CTNAG 3' GANTC |
5' ---C TNAG--- 3' 3' ---GANT C--- 5' |
|
BstDSI |
|
Bacillus stearothermophilus DS |
5' CCRYGG 3' GGYRCC |
5' ---C CRYGG--- 3' 3' ---GGYRC C--- 5' |
|
BstEII |
|
Bacillus stearothermophilus ET |
5' GGTNACC 3' CCANTGG |
5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' |
AcrII, BstPI, BstT9I, BstT10I, EcaI, Eco91I, EcoO65I, NspSAII, PspEI |
BstENI |
|
Bacillus stearothermophilus EN |
5' CCTN4NAGG 3' GGAN4NTCC |
5' ---CCTNN NNNAGG--- 3' 3' ---GGANNN NNTCC--- 5' |
|
BstENII |
|
Bacillus stearothermophilus EN |
5' GATC 3' CTAG |
5' --- GATC--- 3' 3' ---CTAG --- 5' |
BfuCI, Bsp2095I, BspKT6I, CcyI, FnuCI, MboI, NmeCI, SsiAI |
BstEZ359I |
|
Bacillus stearothermophilus EZ359 |
5' GTTAAC 3' CAATTG |
5' ---GTT AAC--- 3' 3' ---CAA TTG--- 5' |
|
BstFI |
|
Bacillus stearothermophilus FH58 |
5' AAGCTT 3' TTCGAA |
5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' |
|
BstF5I |
|
Bacillus stearothermophilus F5 |
5' GGATG 3' CCTAC |
5' ---GGATGNN --- 3' 3' ---CCTAC NN--- 5' |
|
BstFNI |
|
Bacillus stearothermophilus |
5' CGCG 3' GCGC |
5' ---CG CG--- 3' 3' ---GC GC--- 5' |
AccII, BceBI, BepI, Bpu95I, BstUI, Csp68KVI, FauBII, MvnI, SelI |
BstFZ438I |
|
Bacillus stearothermophilus FZ438 |
5' CCCGC 3' GGGCG |
5' ---CCCGCNNNN NN--- 3' 3' ---GGGCGNNNNNN --- 5' |
|
BstGZ53I |
|
Bacillus stearothermophilus GZ53 |
5' CGTCTC 3' GCAGAG |
5' ---CGTCTCN NNNN--- 3' 3' ---GCAGAGNNNNN --- 5' |
|
BstH2I |
|
Bacillus stearothermophilus H2 |
5' RGCGCY 3' YCGCGR |
5' ---RGCGC Y--- 3' 3' ---Y CGCGR--- 5' |
AccB2I, |
BfoI, |
Bme142I, |
Bsp143II, HaeII, LpnI |
|
BstH9I |
|
Bacillus stearothermophilus H9 |
5' GGATC 3' CCTAG |
5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5' |
AclWI, AlwI, BinI, |
BpuFI, |
BspPI, |
Bst31TI, BthII, Bth617I, |
EacI |
|
BstHHI |
|
Bacillus stearothermophilus HH |
5' GCGC 3' CGCG |
5' ---GCG C--- 3' 3' ---C GCG--- 5' |
AspLEI, BspLAI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI |
BstHPI |
|
Bacillus stearothermophilus HP |
5' GTTAAC 3' CAATTG |
5' ---GTT AAC--- 3' 3' ---CAA TTG--- 5' |
|
BstHZ55I |
|
Bacillus stearothermophilus HZ55 |
5' CCAN6TGG 3' GGTN6ACC |
5' ---CCANNNNN NTGG--- 3' 3' ---GGTN NNNNNACC--- 5' |
|
BstIZ316I |
|
Bacillus stearothermophilus IZ316 |
5' CACNNNGTG 3' GTGNNNCAC |
5' ---CACNNN GTG--- 3' 3' ---GTG NNNCAC--- 5' |
AdeI, DraIII |
BstJZ301I |
|
Bacillus stearothermophilus JZ301 |
5' CTNAG 3' GANTC |
5' ---C TNAG--- 3' 3' ---GANT C--- 5' |
|
BstKTI |
|
Bacillus stearothermophilus KT |
5' GATC 3' CTAG |
5' ---GAT C--- 3' 3' ---C TAG--- 5' |
AspMDI, Bsp2095I, Bst19II, BspKT6I, DpnII, MboI, NmeCI, SsiAI |
BstM6I |
|
Bacillus stearothermophilus M6 |
5' CCWGG 3' GGWCC |
5' ---CC WGG--- 3' 3' ---GGW CC--- 5' |
AorI, BseBI, Bse24I, BstNI, BstOI, Bst2UI, BthDI, Fsp1604I, MvaI |
BstMAI |
|
Bacillus stearothermophilus MA |
5' GTCTC 3' CAGAG |
5' ---GTCTCN NNNNN--- 3' 3' ---CAGAGNNNNN N--- 5' |
Alw26I, |
BcoDI, BscQII, |
BsmAI, |
BsoMAI |
|
BstMBI |
|
Bacillus stearothermophilus MB |
5' GATC 3' CTAG |
5' --- GATC--- 3' 3' ---CTAG --- 5' |
Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, NdeII, Sth368I |
BstMCI |
|
Bacillus stearothermophilus MC |
5' CGRYCG 3' GCYRGC |
5' ---CGRY CG--- 3' 3' ---GC YRGC--- 5' |
|
BstMWI |
|
Bacillus stearothermophilus MW |
5' GCN7GC 3' CGN7CG |
5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5' |
|
BstMZ611I |
|
Bacillus stearothermophilus MZ611 |
5' CCNGG 3' GGNCC |
5' --- CCNGG--- 3' 3' ---GGNCC --- 5' |
|
BstNI |
|
Bacillus stearothermophilus |
5' CCWGG 3' GGWCC |
5' ---CC WGG--- 3' 3' ---GGW CC--- 5' |
AjnI, BciBII, BptI, Bst1I, BstOI, Bst2UI, Fsp1604I, SniI, Sth117I |
Bst31NI |
|
Bacillus stearothermophilus 31N |
5' CCGCTC 3' GGCGAG |
5' ---CCG CTC--- 3' 3' ---GGC GAG--- 5' |
AccBSI, BsrBI, BstD102I, MbiI |
BstNSI |
|
Bacillus stearothermophilus 1161NS |
5' RCATGY 3' YGTACR |
5' ---RCATG Y--- 3' 3' ---Y GTACR--- 5' |
|
BstNZ169I |
|
Bacillus stearothermophilus NZ169 |
5' ATCGAT 3' TAGCTA |
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5' |
BanIII, BciBI, BdiI, Bli41I, Bsa29I, BspDI, LplI, Rme21I, SpmI, ZhoI |
BstOI |
|
Bacillus stearothermophilus O22 |
5' CCWGG 3' GGWCC |
5' ---CC WGG--- 3' 3' ---GGW CC--- 5' |
AjnI, ApyI, BciBII, BptI, Bst1I, Bst2UI, Fsp1604I, SniI, Sth117I |
BstOZ616I |
|
Bacillus stearothermophilus OZ616 |
5' GGGAC 3' CCCTG |
5' ---GGGACN8NN NNNN--- 3' 3' ---CCCTGN8NNNNNN --- 5' |
|
BstPI |
|
Bacillus stearothermophilus |
5' GGTNACC 3' CCANTGG |
5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' |
AcrII, AspAI, BstEII, BstT9I, EcaI, Eco91I, EcoO65I, NspSAII, PspEI |
BstPAI |
|
Bacillus stearothermophilus PA |
5' GACN4GTC 3' CTGN4CAG |
5' ---GACNN NNGTC--- 3' 3' ---CTGNN NNCAG--- 5' |
|
BstPZ418I |
|
|
5' GGATG 3' CCTAC |
5' ---GGATGN8N NNNN--- 3' 3' ---CCTACN8NNNNN --- 5' |
|
BstPZ740I |
|
Bacillus stearothermophilus PZ740 |
5' CTTAAG 3' GAATTC |
5' ---C TTAAG--- 3' 3' ---GAATT C--- 5' |
|
BstRZ246I |
|
Bacillus stearothermophilus RZ246 |
5' ATTTAAAT 3' TAAATTTA |
5' ---ATTT AAAT--- 3' 3' ---TAAA TTTA--- 5' |
|
BstSI |
|
Bacillus stearothermophilus S183 |
5' CYCGRG 3' GRGCYC |
5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5' |
Ama87I, AvaI, Bse15I, BsoBI, Eco27kI, NspIII, OfoI, PunAI |
BstSCI |
|
Bacillus stearothermophilus SC |
5' CCNGG 3' GGNCC |
5' --- CCNGG--- 3' 3' ---GGNCC --- 5' |
|
BstSFI |
|
Bacillus stearothermophilus SF |
5' CTRYAG 3' GAYRTC |
5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5' |
|
BstSNI |
|
Bacillus stearothermophilus SN |
5' TACGTA 3' ATGCAT |
5' ---TAC GTA--- 3' 3' ---ATG CAT--- 5' |
|
BstSWI |
|
Bacillus stearothermophilus SW |
5' ATTTAAAT 3' TAAATTTA |
5' ---ATTT AAAT--- 3' 3' ---TAAA TTTA--- 5' |
|
BstT7I |
|
Bacillus stearothermophilus T7 |
5' TGATCA 3' ACTAGT |
5' ---T GATCA--- 3' 3' ---ACTAG T--- 5' |
AbaI, BclI, BsiQI, |
BspXII, FbaI, Ksp22I, |
ParI |
|
BstT9I |
|
Bacillus stearothermophilus T9 |
5' GGTNACC 3' CCANTGG |
5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' |
AspAI, Bse64I, BstT10I, BstPI, Eco91I, EcoO128I, NspSAII, PspEI |
BstT10I |
|
Bacillus stearothermophilus T10 |
5' GGTNACC 3' CCANTGG |
5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5' |
AspAI, BseT10I, BstEII, BstT10I, Eco91I, EcoO128I, NspSAII, PspEI |
Bst31TI |
|
Bacillus stearothermophilus 31T |
5' GGATC 3' CCTAG |
5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5' |
|
BstTS5I |
|
Bacillus stearothermophilus TS5 |
5' GAAGAC 3' CTTCTG |
5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' |
|
BstUI |
|
Bacillus stearothermophilus U458 |
5' CGCG 3' GCGC |
5' ---CG CG--- 3' 3' ---GC GC--- 5' |
AccII, BceBI, BepI, Bpu95I, BtkI, Csp68KVI, FauBII, MvnI, SelI |
Bst2UI |
|
Bacillus stearothermophilus 2U |
5' CCWGG 3' GGWCC |
5' ---CC WGG--- 3' 3' ---GGW CC--- 5' |
AjnI, BciBII, BptI, Bst1I, BstOI, BstM6I, SniI, SslI, TaqXI, ZanI |
BstVI |
|
Bacillus stearothermophilus V |
5' CTCGAG 3' GAGCTC |
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5' |
BluI, BssHI, MlaAI, PanI, SauLPII, SciI, SlaI, StrI, XhoI |
BstV1I |
|
Bacillus stearothermophilus V1 |
5' GCAGC 3' CGTCG |
5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5' |
AlwXI, BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I |
BstV2I |
|
Bacillus stearothermophilus V2 |
5' GAAGAC 3' CTTCTG |
5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5' |
|
BstXI |
|
Bacillus stearothermophilus X1 |
5' CCAN6TGG 3' GGTN6ACC |
5' ---CCANNNNN NTGG--- 3' 3' ---GGTN NNNNNACC--- 5' |
|
BstX2I |
|
Bacillus stearothermophilus X2 |
5' RGATCY 3' YCTAGR |
5' ---R GATCY--- 3' 3' ---YCTAG R--- 5' |
|
BstYI |
1SDO |
Bacillus stearothermophilus Y406 |
5' RGATCY 3' YCTAGR |
5' ---R GATCY--- 3' 3' ---YCTAG R--- 5' |
|
BstZI |
|
Bacillus stearothermophilus Z130 |
5' CGGCCG 3' GCCGGC |
5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5' |
AaaI, BseX3I, EagI, EclXI, Eco52I, SenPT16I, XmaIII |
BstZ17I |
|
Bacillus stearothermophilus 38M |
5' GTATAC 3' CATATG |
5' ---GTA TAC--- 3' 3' ---CAT ATG--- 5' |
|